Measurements ofGValues for DNA Damage Induced by Low-Energy Electrons
نویسندگان
چکیده
منابع مشابه
Damage induced to DNA by low-energy (0-30 eV) electrons under vacuum and atmospheric conditions.
In this study, we show that it is possible to obtain data on DNA damage induced by low-energy (0-30 eV) electrons under atmospheric conditions. Five monolayer films of plasmid DNA (3197 base pairs) deposited on glass and gold substrates are irradiated with 1.5 keV X-rays in ultrahigh vacuum and under atmospheric conditions. The total damage is analyzed by agarose gel electrophoresis. The damage...
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Thin films of the short single strand of DNA, GCAT, in which guanine (G) or adenine (A) have been removed, were bombarded under vacuum by 4 to 15 eV electrons. The fragments corresponding to base release and strand breaks (SB) were analyzed by high performance liquid chromatography and their yields compared with those obtained from unmodified GCAT. From such a comparison, it is shown that, usin...
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The damage induced to a model DNA (dT25) immobilized on a gold surface by the interaction of low-energy (1 eV) electrons was studied by means of microarray technology. High quality single-stranded DNA arrays were hybridized with a dye-marked complementary strand after irradiation with electrons and the normalized fluorescence data were used to quantify the DNA damage. The data clearly show the ...
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We report results on the desorption of OH- induced by 0-19 eV electrons incident on self-assembled monolayer films made of single and double DNA strands of different orientations with respect to a gold substrate. Such measurements make it possible to deduce the mechanism and site of OH- formation within a biomolecule as complex as DNA. This type of damage is attributed to dissociative electron ...
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Low-energy electrons (LEEs) are produced in large quantities in any type of material irradiated by high-energy particles. In biological media, these electrons can fragment molecules and lead to the formation of highly reactive radicals and ions. The results of recent experiments performed on biomolecular films bombarded with LEEs under ultra-high vacuum conditions are reviewed in the present ar...
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ژورنال
عنوان ژورنال: The Journal of Physical Chemistry B
سال: 2011
ISSN: 1520-6106,1520-5207
DOI: 10.1021/jp207922n